The Histopathology Department is a tertiary referral Centre for Oncopathology and Immunofluorescence microscopy.
Facilities available in the Department are as follows:
- Histopathology- Reporting Biopsies, surgical pathology specimens & special stains.
- Intraoperative Frozen Sections – / STEROTACTIC BRAIN BIOPSIES on cryostat.
- Liquid based Cytology – for detection of malignant cells in body fluids.
- Fine Needle Aspiration Cytology (FNAC) – The procedure in performed on swellings in breast/Thyroid/Lymph nodes etc.
- Liquid based Cytology for Gynaecological – PAP Smears-For Early detection of Cervical Cancer.
- Immunohistochemistry – more than 100 tumour markers are available in the Department. These markers are done on formalin fixed tissue in paraffin blocks. These markers help in diagnosis, classification and prognosticastion of various Cancers.
- Immunofluorescence Microscopy – For evaluation of kidney and skin biopsies.Test Panel: IgG, IgA, IgM, C3, C1q, Fibrinogen, Kappa, Lamda
- Cytogenetics – Fluorescent in Situ Hybridisation (FISH) technique for Her2/neu testing in breast Cancer.
Important Instructions
- For Direct Immunofluorescence: Fresh biopsy to be collected in Michel’s Transport Medium provided by the Histopathology Department.
- If there is a time lag between collection and delivery to the histopathology lab, kindly preserve sample at 2-8 deg.C in the refrigerator.
Panels
Non Hodgkin’s Lymphoma Panel
Alk1, BCL2, BCL6, CD3, CD5, CD10, CD20, CD21, CD23, CD43, CD45, CD68, CD79a, CyclinD, Pax5, TdT,Ki67
Small round cell tumor Panel
CD99, Desmin, TdT, LCA, Myogenin, Fli-1, Synaptophysin, Chromogranin, WT1
Metastatic Panel/Carcinoma of Unknown Primary (CUP)
CK7,CK20, CEA, CDX2,TTF-1, Thyroglobulin,WT1,Vimentin,PSA/PSAP, Synaptophysin/Chromogranin,S100/HMB-45
Immunohistochemistry
Various marker panels are available.
Breast Panel
ER, PR, Her2neu, Ki67, GCDFP15, E Cadherin
Hodgkin’s Lymphoma Panel
LCA, CD3, CD20, CD15, CD30, PAX5
Germ cell tumor Panel
AFP, B-HCG, PLAP
Ovarian Panel
CA125, CEA, Inhibin-Alpha
Prostate Panel
PSA, PSAP, AMACR, HMWCK, P63
Thyroid Pane
Thyroglobulin, Calcitonin, TTF1, HBME-1
Lung Panel
NapsinA, TTF1, CK7, BerEP4
Kidney Panel
C4d, CD10, Wilm’s Tumor (WT-1)
Colon / Gastric Panel
CDX2, CK7, CK20,
MSI Panel
MLH1, MSH2, MSH6, PMS2
Neuroendocrine Panel
NSE, Chromogranin, Synaptopysin
Melanoma Panel
HMB45, MelanA, S100
Carcinoma Panel
Cytokeratin, CK7, CK8+18, CK20, HMWCK, EMA, CEA, LMWCK
Sarcoma Panel
SMA, Desmin, CD34, S100, Vimentin, CD117
Liver Panel
Glypican 3, HSA, AFP.
GIST Panel
C-Kit, DOG1,SMA,S100, CD34
Myoepithelial Panel
Calponin, P63, SMA, SMM
Plasma cell Panel
CD138, Kappa, Lambda
Pancreatic Panel
CA19.9,CK7,CK20