The Histopathology Department is a tertiary referral Centre for Oncopathology and Immunofluorescence microscopy.

Facilities available in the Department are as follows:

  • Histopathology- Reporting Biopsies, surgical pathology specimens & special stains.
  • Intraoperative Frozen Sections – / STEROTACTIC BRAIN BIOPSIES on cryostat.
  • Liquid based Cytology – for detection of malignant cells in body fluids.
  • Fine Needle Aspiration Cytology (FNAC) – The procedure in performed on swellings in breast/Thyroid/Lymph nodes etc.
  • Liquid based Cytology for Gynaecological – PAP Smears-For Early detection of Cervical Cancer.
  • Immunohistochemistry – more than 100 tumour markers are available in the Department. These markers are done on formalin fixed tissue in paraffin blocks. These markers help in diagnosis, classification and prognosticastion of various Cancers.
  • Immunofluorescence Microscopy – For evaluation of kidney and skin biopsies.Test Panel: IgG, IgA, IgM, C3, C1q, Fibrinogen, Kappa, Lamda
  • Cytogenetics – Fluorescent in Situ Hybridisation (FISH) technique for Her2/neu testing in breast Cancer.

Important Instructions

  • For Direct Immunofluorescence: Fresh biopsy to be collected in Michel’s Transport Medium provided by the Histopathology Department.
  • If there is a time lag between collection and delivery to the histopathology lab, kindly preserve sample at 2-8 deg.C in the refrigerator.

Panels

Non Hodgkin’s Lymphoma Panel

Alk1, BCL2, BCL6, CD3, CD5, CD10, CD20, CD21, CD23, CD43, CD45, CD68, CD79a, CyclinD, Pax5, TdT,Ki67

Small round cell tumor Panel

CD99, Desmin, TdT, LCA, Myogenin, Fli-1, Synaptophysin, Chromogranin, WT1

Metastatic Panel/Carcinoma of Unknown Primary (CUP)

CK7,CK20, CEA, CDX2,TTF-1, Thyroglobulin,WT1,Vimentin,PSA/PSAP, Synaptophysin/Chromogranin,S100/HMB-45

Immunohistochemistry

Various marker panels are available.

Breast Panel

ER, PR, Her2neu, Ki67, GCDFP15, E Cadherin

Hodgkin’s Lymphoma Panel

LCA, CD3, CD20, CD15, CD30, PAX5

Germ cell tumor Panel

AFP, B-HCG, PLAP

Ovarian Panel

CA125, CEA, Inhibin-Alpha

Prostate Panel

PSA, PSAP, AMACR, HMWCK, P63

Thyroid Pane

Thyroglobulin, Calcitonin, TTF1, HBME-1

Lung Panel

NapsinA, TTF1, CK7, BerEP4

Kidney Panel

C4d, CD10, Wilm’s Tumor (WT-1)

Colon / Gastric Panel

CDX2, CK7, CK20,

MSI Panel

MLH1, MSH2, MSH6, PMS2

Neuroendocrine Panel

NSE, Chromogranin, Synaptopysin

Melanoma Panel

HMB45, MelanA, S100

Carcinoma Panel

Cytokeratin, CK7, CK8+18, CK20, HMWCK, EMA, CEA, LMWCK

Sarcoma Panel

SMA, Desmin, CD34, S100, Vimentin, CD117

Liver Panel

Glypican 3, HSA, AFP.

GIST Panel

C-Kit, DOG1,SMA,S100, CD34

Myoepithelial Panel

Calponin, P63, SMA, SMM

Plasma cell Panel

CD138, Kappa, Lambda

Pancreatic Panel

CA19.9,CK7,CK20